Microorganism tracking microscope
Continuous observation of individual motile microorganisms is difficult, since their swimming speed is fast compared with their diameters. Microorganisms can quickly go out of the range of static measurement instruments, such as the field of view of optical microscopes.
To solve this problem, a microorganism tracking system using a high-speed vision system has been developed. This system tracks a freely swimming microorganism within the field of an optical microscope by moving a chamber of target microorganisms based on high-speed visual feedback.
A freely swimming Ascidian Spermetozoa is tracked at the center of the field of view
Result of 3-D tracking of a paramecium
* Cited from Ishikawa Watanabe Lab. Channel, where the PI of this lab. belonged until March 2014.
Fig. System connection of the tracking microscope system (left), and an image sequence of a paramecium which is 3-D tracked.
References
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Hiromasa Oku, Naoko Ogawa, Kogiku Shiba, Manabu Yoshida, and Masatoshi Ishikawa : How to Track Spermatozoa using High-Speed Visual Feedback, 30th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2008) (Vancouver, 2008.8.21) / Proceedings, pp.125-128 (Oral)
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Hiromasa Oku, Masatoshi Ishikawa, Theodorus, and Koichi Hashimoto : High-speed autofocusing of a cell using diffraction pattern, Optics Express 14, pp.3952-3960 (2006) http://www.opticsinfobase.org/abstract.cfm?URI=oe-14-9-3952
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Hiromasa Oku, Naoko Ogawa, Koichi Hashimoto, and Masatoshi Ishikawa : Two-dimensional tracking of a motile microorganism allowing high-resolution observation with various imaging techniques, Review of Scientific Instruments, Vol.76, No.3, 034301 (2005)
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Hiromasa Oku, Idaku Ishii, and Masatoshi Ishikawa : A Dynamic Image Control System, Systems and Computers in Japan, Vol., No.13, pp.71-79 (2004)
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Hiromasa OKU, Idaku ISHII, and Masatoshi ISHIKAWA : Tracking a Protozoon Using High-Speed Visual Feedback, Proc. of 1st Annual Int. IEEE-EMBS Special Topic Conf, on Microtechnologies in Medicine & Biology(Lyon, 2000.10.12-14)/pp.156-159